Reveal the immune specificity with complete V(D)J sequences
SeekOneDD single-cell immune profiling kit (5' transcriptome & immune repertoire sequencing), manufactured by Beijing SeekGene BioSciences Co., Ltd., achieves single-cell partitioning and labelling by the microfluidic system and barcoded beads. The library of both full-length TCR/BCR sequences and mRNA sequences can be constructed from a single sample, followed by high-throughput sequencing and bioinformatics analysis.
The SeekOne DD Single Cell Immune Profiling Kit includes the SeekOneDD Single Cell 5’ Transcriptome-seq Kit and the SeekOneDD TCR/BCR amplification Kit. With this kit, the full-length sequence of V(D)J, the pairing information of α chain (heavy chain) and β chain (light chain) of TCR(BCR) and the transcriptome information can be obtained simultaneously. It can be used to investigate T/B cell heterogeneity, TCR/BCR diversity, antigen specificity, vaccine development, immunetherapy screening, etc.
SeekOneDD single cell immune profiling products are based on the principle of microfluidic technology. Cells and barcoded beads are added separately and then reacted in the carrier oil to form emulsion droplets in the shaped channel. After capturing mRNA tagged with barcoded beads, two separate libraries (gene expression & immune repertoire) can be constructed from a single sample.