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SeekGene SeekOne DD System

Waren-Nr. PSD1
26×21.5×23 cm, 7.25 kg
Digital Droplet

Product Information

Feel life with speed

SeekOneDigital Droplet System (SeekOneDD System), self-developed by Beijing SeekGene BioSciences Co., Ltd., achieves single-cell partitioning and capture by water-in-oil droplets generated in the microfluidic system. This provides a one-stop shop for single-cell solutions, from single-cell partitioning, trapping, labelling and library construction to data analysis. It can be applied to a variety of medical studies, including cancer, immunity, cell development, viral infection, drug discovery, and target screening.

Based on microfluidic technology, the system achieves single-cell partitioning and capture with water-in-oil droplets. Barcoded beads are designed for the molecular labelling of different cells and their mRNAs. After labelling single cells for gene expression, high-throughput single-cell libraries can be constructed. Finally, SeekSoul® Tools, our efficient single-cell analysis software, can be used to process and analyze single-cell data.

 

Core technology

 

Cells and barcoded beads are added separately and then react in the carrier oil to form emulsion droplets in the shaped channel. After that, mRNA molecules released by the cells are captured by oligo(dT) on the barcoded beads.

Product Features

Fast&Accurate
Affordable
Compatible
User-friendly

Workflow

Step. 1
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1. Sample transport
Step. 2
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2. Tissue dissociation
Step. 3
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3. Single cell separation and labeling
Step. 4
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4. Library construction
Step. 5
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5. NGS sequencing
Step. 6
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6. Bioinformatic analysis
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Overview

Flexible cell diameter of 5~40 μm
Rapid generation of 150000 water-in-oil droplets in 3 minutes
Efficiently capture 500-12,000 cells per channel
Flexible running of 1~8 samples in parallel
High cell capture rates of up to 65%
Low doublet rates of under 0.3% per 1,000 cells
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Content

1 × SeekOneDD Chip Holder
8 × Placed Chip
Compatible with SeekOne DD Single Cell 3' Transcriptome Kit
Compatible with SeekOne DD Single Cell Immune Profiling Kit
Compatible with SeekOne DD Single Cell scFAST-seq Kit

Product name
SeekOne® Digital Droplet System
Manufacturer
SeekGene BioSciences Co., Ltd.
Catalog no.
M001A
Others
For Research Use Only. Not for use in diagnostic procedures.

Documents

Brochure-SeekOne_DD_System.pdf
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