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SeekOneDigital Droplet System (SeekOneDD System), self-developed by Beijing SeekGene BioSciences Co., Ltd., achieves single-cell partitioning and capture by water-in-oil droplets generated in the microfluidic system. This provides a one-stop shop for single-cell solutions, from single-cell partitioning, trapping, labelling and library construction to data analysis. It can be applied to a variety of medical studies, including cancer, immunity, cell development, viral infection, drug discovery, and target screening.
Based on microfluidic technology, the system achieves single-cell partitioning and capture with water-in-oil droplets. Barcoded beads are designed for the molecular labelling of different cells and their mRNAs. After labelling single cells for gene expression, high-throughput single-cell libraries can be constructed. Finally, SeekSoul® Tools, our efficient single-cell analysis software, can be used to process and analyze single-cell data.
Cells and barcoded beads are added separately and then react in the carrier oil to form emulsion droplets in the shaped channel. After that, mRNA molecules released by the cells are captured by oligo(dT) on the barcoded beads.