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SeekGene SeekOne DD Single Cell 3' Transcriptome kit

Waren-Nr. PST1
8 Tests/Kit
3' Transcriptome-seq

Product Information

Investigate gene expression with accuracy and efficiency

SeekOneDD (Digital Droplet) Single Cell 3' Transcriptome-seq is a high-throughput single-cell RNA-seq library kit which achieves single-cell partitioning and labelling by the microfluidic system and barcoded beads.

The kit needs to be used with the SeekOneDigital Droplet System (SeekOneDD) to complete the whole process from single-cell transcriptome barcoding to library construction.

Based on the principle of microfluidic technology, the kit uses emulsion droplets to separate and capture individual cells, and then recruits nucleic acid-modified Barcoded Beads to molecularly label RNA from different cell sources.

Finally, a high-throughput single-cell transcriptome library compatible with Illumina and MGI sequencing system will be constructed, which can be used for research on tumors, immunity, cell development, viral infections, drug discovery, and target screening, etc.

 

Core Technology

 

The core technology of SeekOne® DD Single Cell 3’ Transcriptome-seq. Cells and barcoded beads are added separately and then react in the carrier oil to form emulsion droplets in the shaped channel. After that, mRNA molecules released by the cells are captured by oligo(dT) on the barcoded beads.

Product Features

Compatible
Fast
Affordable
Versatile

Workflow

Step. 1
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1. Sample transport
Step. 2
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2. Tissue dissociation
Step. 3
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3. Single cell separation and labeling
Step. 4
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4. Library construction
Step. 5
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5. NGS sequencing
Step. 6
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6. Bioinformatic analysis
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Overview

1-8 samples can be flexibly run at a time
500-12,000 cells can be captured with a single channel
150,000 emulsion droplets can be generated in 3 minutes
High cell capture rates of up to 65%
Doublet rate approximately 0.3% per 1000 cells
Cell aggregation rate<10%
Nucleated cell rate>70%
Cell lysis and labeling experiments within 2 hours of placing the sample on ice
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Content

1 × SeekOne® DD Chip S3 Kit for 8 tests
1 × SeekOne® DD Single Cell 3’ Barcoded Beads Kit for 8 tests
1 × SeekOne® DD Single Cell 3’ Reverse Transcription Kit for 8 tests
1 × SeaekOne® DD Library Construction Kit for 8 tests
1 × SeekOne® DD Single Cell Cleanup Kit for 8 tests

Product name
SeekOne DD Single Cell 3' Transcriptome kit
Manufacturer
SeekGene BioSciences Co., Ltd.
Catalog no.
K00202-08
Others
For Research Use Only. Not for use in diagnostic procedures.

Documents

Brochure-SeekOne_DD_sc3_RNA_seq.pdf
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